Immunofluorescent analysis of HepG2 cells using CSB-PA018656LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA018656LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA018656LA01HU at dilution of 1:100
Immunoprecipitating PRDX3 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA018656LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA018656LA01HU (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse stomach tissue All lanes: PRDX3 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28, 26 kDa Observed band size: 28 kDa
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