P4HA2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA017340ESR1HU
Article Name: P4HA2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA017340ESR1HU
Supplier Catalog Number: CSB-PA017340ESR1HU
Alternative Catalog Number: CSB-PA017340ESR1HU-100UL, CSB-PA017340ESR1HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, IP, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: 2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody, 4 PH alpha 2 antibody, 4-PH alpha-2 antibody, C-P4Halpha(II) antibody, collagen prolyl 4-hydroxylase alpha(II) antibody, P4HA2 antibody, P4HA2_HUMAN antibody, procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II antibody, procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2 antibody, Procollagen-proline antibody, procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody, Prolyl 4 hydroxylase alpha 2 subunit antibody, Prolyl 4 hydroxylase subunit alpha 2 [Precursor] antibody, Prolyl 4-hydroxylase subunit alpha-2 antibody, Prolyl 4-hydroxylase, alpha polypeptide II antibody
Clonality: Polyclonal
UniProt: O15460
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purity: Antigen Affinity Purified
Form: Liquid
Target: P4HA2
Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA017340ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA017340ESR1HU at dilution of 1:100
Immunoprecipitating P4HA2 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA017340ESR1HU in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA017340ESR1HU (4ug) + Hela whole cell lysate (500ug)
Lane 3: Hela whole cell lysate (20ug)
Western blot
All lanes: P4HA2 antibody at 6ug/ml
Lane 1: HepG2 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa