OVOL1 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA017303LA01HU
- Images (4)
| Article Name: | OVOL1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA017303LA01HU |
| Supplier Catalog Number: | CSB-PA017303LA01HU |
| Alternative Catalog Number: | CSB-PA017303LA01HU-100UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC, WB |
| Species Reactivity: | Human, Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | hOvo1 antibody, Ovo homolog like 1 antibody, Ovo like 1(Drosophila) antibody, Ovo, Drosophila, homolog-like, 1 antibody, Ovol1 antibody, OVOL1_HUMAN antibody, Putative transcription factor Ovo like 1 antibody, Putative transcription factor Ovo-like 1 antibody |
| Clonality: | Polyclonal |
| UniProt: | O14753 |
| Buffer: | Preservative: 0.02% sodium azide<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | OVOL1 |
| Application Dilute: | Recommended dilution: WB:1:1000-1:3000, IHC:1:50-1:200 |
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Immunohistochemistry of paraffin-em |
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IHC image of CSB-PA017303LA01HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody |
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IHC image of CSB-PA017303LA01HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody |
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Western Blot Positive WB detected in: U251 whole cell lysate(20µg), MCF7 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), 293T whole cell lysate(20µg), A549 whole cell lysate(20µg),Mouse Brain tissue lysate(20µg) All lanes: OVOL1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 30s |
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