OLR1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA016331LA01HU
Article Name: OLR1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA016331LA01HU
Supplier Catalog Number: CSB-PA016331LA01HU
Alternative Catalog Number: CSB-PA016331LA01HU-100UG, CSB-PA016331LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Rat
Conjugation: Unconjugated
Alternative Names: C-type lectin domain family 8 member A antibody, CLEC8A antibody, hLOX 1 antibody, hLOX-1 antibody, Lectin like oxidized LDL receptor 1 antibody, Lectin like oxLDL receptor 1 antibody, Lectin type oxidized LDL receptor 1 antibody, Lectin-like oxidized LDL receptor 1 antibody, Lectin-like oxLDL receptor 1 antibody, Lectin-type oxidized LDL receptor 1 antibody, low density lipoprotein oxidized, receptor 1 antibody, LOX-1 antibody, LOXIN antibody, Olr1 antibody, OLR1_HUMAN antibody, Ox LDL receptor 1 antibody, Ox-LDL receptor 1 antibody, Oxidised low density lipoprotein (lectin like) receptor 1 antibody, Oxidized low density lipoprotein receptor 1 antibody, Oxidized low density lipoprotein receptor 1 soluble form antibody, Oxidized low-density lipoprotein receptor 1 antibody, OxLDL receptor 1 antibody, SCARE1 antibody, Scavenger receptor class E, member 1 antibody, SLOX1 antibody, soluble form antibody, SR-EI antibody
Clonality: Polyclonal
UniProt: P78380
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: OLR1
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500
Immunofluorescence staining of A549 cells with CSB-PA016331LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA016331LA01HU diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA016331LA01HU diluted at 1:1200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot
Positive WB detected in: Rat kidney tissue
All lanes: OLR1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31, 21, 22 kDa
Observed band size: 31 kDa