Immunofluorescent analysis of HepG2 cells using CSB-PA016059LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA016059LA01HU at dilution of 1:100
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: NR3C1 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 82, 65, 61, 83, 79, 77, 76, 52, 51, 50 kDa Observed band size: 86 kDa
Immunoprecipitating NR3C1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA016059LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: CSB-PA016059LA01HU (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
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