NR3C1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA016059LA01HU
Article Name: NR3C1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA016059LA01HU
Supplier Catalog Number: CSB-PA016059LA01HU
Alternative Catalog Number: CSB-PA016059LA01HU-100UG, CSB-PA016059LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, IP, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: GCCR antibody, GCR antibody, GCR_HUMAN antibody, GCRST antibody, glucocorticoid nuclear receptor variant 1 antibody, Glucocorticoid receptor antibody, GR antibody, GRL antibody, Grl1 antibody, nr3c1 antibody, Nuclear receptor subfamily 3 group C member 1 antibody, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
Clonality: Polyclonal
UniProt: P04150
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: NR3C1
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Immunofluorescent analysis of HepG2 cells using CSB-PA016059LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA016059LA01HU at dilution of 1:100
Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: NR3C1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86, 82, 65, 61, 83, 79, 77, 76, 52, 51, 50 kDa
Observed band size: 86 kDa
Immunoprecipitating NR3C1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA016059LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA016059LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)