NMU Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA015902ESR1HU
Article Name: NMU Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA015902ESR1HU
Supplier Catalog Number: CSB-PA015902ESR1HU
Alternative Catalog Number: CSB-PA015902ESR1HU-100UL, CSB-PA015902ESR1HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: Neuromedin U 25 antibody, Neuromedin U antibody, Neuromedin-U-25 antibody, NmU 25 antibody, NMU antibody, NmU-25 antibody, NMU_HUMAN antibody
Clonality: Polyclonal
UniProt: P48645
Buffer: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4.
Purity: Antigen Affinity Purified
Form: Liquid
Target: NMU
Application Dilute: Recommended dilution: WB:1:500-1:2000,IF:1:20-1:100
Immunohistochemistry of paraffi
Immunofluorescence staining of Hela cell with CSB-PA015902ESR1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: HepG2 whole cell lysate(30µg),Mouse Stomach tissue lysate(30µg),Mouse Small intestine tissue lysate(30µg)
All lanes: NMU antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 120s