PSMB2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA01385A0RB
Article Name: PSMB2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA01385A0RB
Supplier Catalog Number: CSB-PA01385A0Rb
Alternative Catalog Number: CSB-PA01385A0RB-100UG, CSB-PA01385A0RB-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: HC7 I antibody, Macropain subunit C7 I antibody, Macropain subunit C7-I antibody, Multicatalytic endopeptidase complex subunit C7 1 antibody, Multicatalytic endopeptidase complex subunit C7 I antibody, Multicatalytic endopeptidase complex subunit C7-I antibody, Proteasome (prosome, macropain) subunit beta type 2 antibody, Proteasome beta 2 subunit antibody, Proteasome component C7 I antibody, Proteasome component C7-I antibody, Proteasome subunit beta type-2 antibody, PSB2_HUMAN antibody, Psmb2 antibody
Clonality: Polyclonal
UniProt: P49721
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: PSMB2
Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Immunofluorescent analysis of PC-3 cells using CSB-PA01385A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IHC image of CSB-PA01385A0Rb diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA01385A0Rb diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA01385A0Rb at dilution of 1:100
Western blot
All lanes: proteasome subunit beta type-2 antibody at 2ug/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa