ITGB6 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA011890LA01HU
Article Name: ITGB6 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA011890LA01HU
Supplier Catalog Number: CSB-PA011890LA01HU
Alternative Catalog Number: CSB-PA011890LA01HU-100UL, CSB-PA011890LA01HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: Integrin beta-6 antibody, ITB6_HUMAN antibody, ITGB6 antibody
Clonality: Polyclonal
UniProt: P18564
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: ITGB6
Application Dilute: Recommended dilution: WB:1:500-1:2000, IF:1:20-1:100
Immunofluorescence staining of A549 cells with CSB-PA011890LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate(30µg), A549 whole cell lysate(30µg), HT29 whole cell lysate(30µg),HEK293 whole cell lysate(30µg)
All lanes: ITGB6 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 86,75 kDa
Observed band size: 86 kDa
Exposure time: 10s