HTR1E Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA010885LA01HU
Article Name: HTR1E Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA010885LA01HU
Supplier Catalog Number: CSB-PA010885LA01HU
Alternative Catalog Number: CSB-PA010885LA01HU-100UG, CSB-PA010885LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: HTR1E5-hydroxytryptamine receptor 1E antibody, 5-HT-1E antibody, 5-HT1E antibody, S31 antibody, Serotonin receptor 1E antibody
Clonality: Polyclonal
UniProt: P28566
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: HTR1E
Application Dilute: Recommended dilution: WB:1:500-1:2000, IF:1:20-1:100
Immunofluorescent analysis of He
Immunofluorescence staining of Hela cell with CSB-PA010885LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: PC-3 whole cell lysate(30µg), HT29 whole cell lysate(30µg)br />All lanes: HTR1E antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 42kDa
Observed band size: 42 kDa
Exposure time:120s