HIST1H3A (Ab-9) Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA010418PA09NACHU
Article Name: HIST1H3A (Ab-9) Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA010418PA09NACHU
Supplier Catalog Number: CSB-PA010418PA09nacHU
Alternative Catalog Number: CSB-PA010418PA09NACHU-100UL, CSB-PA010418PA09NACHU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ChIP, ELISA, IF, IHC, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: H3 histone family member E pseudogene antibody, H3 histone family, member A antibody, H3/A antibody, H31_HUMAN antibody, H3F3 antibody, H3FA antibody, Hist1h3a antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST3H3 antibody, histone 1, H3a antibody, Histone cluster 1, H3a antibody, Histone H3 3 pseudogene antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody
Clonality: Polyclonal
UniProt: P68431
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: HIST1H3A
Application Dilute: Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Immunofluorescent analysis of Hela cells using CSB-PA010418PA09nacHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010418PA09nacHU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010418PA09nacHU at dilution of 1:100
Western Blot
Positive WB detected in: HEK293 cell acid extracts, A375 cell acid extracts, Mouse liver tissue, Mouse brain tissue, Mouse kidney tissue
All lanes: HIST1H3A antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418PA09nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.