Crotonyl-HIST1H2AG (K118) Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA010389PA118CRHU
- Images (4)
| Article Name: | Crotonyl-HIST1H2AG (K118) Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA010389PA118CRHU |
| Supplier Catalog Number: | CSB-PA010389PA118crHU |
| Alternative Catalog Number: | CSB-PA010389PA118CRHU-100UL, CSB-PA010389PA118CRHU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, ELISA, ICC, IF, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | H2AC11 antibody, H2AFP antibody, HIST1H2AG, antibody, H2AC13 antibody, H2AFC antibody, HIST1H2AI, antibody, H2AC15 antibody, H2AFD antibody, HIST1H2AK, antibody, H2AC16 antibody, H2AFI antibody, HIST1H2AL, antibody, H2AC17 antibody, H2AFN antibody, HIST1H2AMHistone H2A type 1 antibody, H2A.1 antibody, Histone H2A/ptl antibody |
| Clonality: | Polyclonal |
| UniProt: | P0C0S8 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | HIST1H2AG |
| Application Dilute: | Recommended dilution: WB:1:200-1:2000, ICC:1:20-1:200, IF:1:50-1:200 |
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Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2AG (CSB-PA010389PA118crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter. |
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Immunofluorescence staining of Hela cells with CSB-PA010389PA118crHU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunocytochemistry analysis of CSB-PA010389PA118crHU diluted at 1:50 and staining in Hela cells (treated with 30mM sodium crotonylate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate (treated by 30mM sodium crotonylate for 4h) All lanes: HIST1H2AG antibody at 0.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa |
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