HIST1H2AG (Ab-9) Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA010389OA09NHIBHU
- Images (4)
| Article Name: | HIST1H2AG (Ab-9) Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA010389OA09NHIBHU |
| Supplier Catalog Number: | CSB-PA010389OA09nhibHU |
| Alternative Catalog Number: | CSB-PA010389OA09NHIBHU-100UL, CSB-PA010389OA09NHIBHU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | H2AC11 antibody, H2AFP antibody, HIST1H2AG, antibody, H2AC13 antibody, H2AFC antibody, HIST1H2AI, antibody, H2AC15 antibody, H2AFD antibody, HIST1H2AK, antibody, H2AC16 antibody, H2AFI antibody, HIST1H2AL, antibody, H2AC17 antibody, H2AFN antibody, HIST1H2AMHistone H2A type 1 antibody, H2A.1 antibody, Histone H2A/ptl antibody |
| Clonality: | Polyclonal |
| UniProt: | P0C0S8 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | HIST1H2AG |
| Application Dilute: | Recommended dilution: WB:1:50-1:500, IHC:1:20-1:200, IF:1:1-1:10 |
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Immunofluorescence staining of Hela cells with CSB-PA010389OA09nhibHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA010389OA09nhibHU diluted at 1:50 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA010389OA09nhibHU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: HIST1H2AG antibody at 1.34µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 32 kDa |
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