HIST1H2AG Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA010389LA01HU
Article Name: HIST1H2AG Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA010389LA01HU
Supplier Catalog Number: CSB-PA010389LA01HU
Alternative Catalog Number: CSB-PA010389LA01HU-100UG, CSB-PA010389LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ChIP, ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: H2AC11 antibody, H2AFP antibody, HIST1H2AG, antibody, H2AC13 antibody, H2AFC antibody, HIST1H2AI, antibody, H2AC15 antibody, H2AFD antibody, HIST1H2AK, antibody, H2AC16 antibody, H2AFI antibody, HIST1H2AL, antibody, H2AC17 antibody, H2AFN antibody, HIST1H2AMHistone H2A type 1 antibody, H2A.1 antibody, Histone H2A/ptl antibody
Clonality: Polyclonal
UniProt: P0C0S8
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: HIST1H2AG
Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Chromatin Immunoprecipitation Hela(1.2*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010389HU) against the PGK1 promoter.
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010389LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010389LA01HU at dilution of 1:100
Western blot
All lanes: HIST1H2AG antibody at 4µg/ml + HL60 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Immunofluorescent analysis of Hela cells using CSB-PA010389LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)