2-hydroxyisobutyryl-HIST1H1C (K109) Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA010378OA109HIBHU
- Images (4)
| Article Name: | 2-hydroxyisobutyryl-HIST1H1C (K109) Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA010378OA109HIBHU |
| Supplier Catalog Number: | CSB-PA010378OA109hibHU |
| Alternative Catalog Number: | CSB-PA010378OA109HIBHU-100UL, CSB-PA010378OA109HIBHU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, ELISA, ICC, IF, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | H1 histone family member 2 antibody, H1.a antibody, H12_HUMAN antibody, H1F2 antibody, H1s-1 antibody, HIST1H1C antibody, Histone 1 H1c antibody, Histone cluster 1 H1c antibody, Histone H1.2 antibody, Histone H1c antibody, Histone H1d antibody, Histone H1s-1 antibody, MGC3992 antibody |
| Clonality: | Polyclonal |
| UniProt: | P16403 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | HIST1H1C |
| Application Dilute: | Recommended dilution: WB:1:100-1:1000, ICC:1:20-1:200, IF:1:20-1:200 |
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Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA010378OA109hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter. |
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Immunocytochemistry analysis of CSB-PA010378OA109hibHU diluted at 1:50 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010378OA109hibHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H1C antibody at 3.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa |
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