H2AFX (Ab-139) Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA010097OA139NPHHU
- Images (5)
| Article Name: | H2AFX (Ab-139) Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA010097OA139NPHHU |
| Supplier Catalog Number: | CSB-PA010097OA139nphHU |
| Alternative Catalog Number: | CSB-PA010097OA139NPHHU-100UL, CSB-PA010097OA139NPHHU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, ELISA, IF, IHC, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | H2A histone family member X antibody, H2A histone family member X antibody, H2A.FX antibody, H2A.X antibody, H2a/x antibody, H2AFX antibody, H2AX antibody, H2AX_HUMAN antibody, Histone H2A.X antibody |
| Clonality: | Polyclonal |
| UniProt: | P16104 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | H2AFX |
| Application Dilute: | Recommended dilution: WB:1:50-1:500, IHC:1:20-1:200, IF:1:1-1:10 |
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFX (CSB-PA010097OA139nphHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter. |
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Immunofluorescence staining of Hela cells with CSB-PA010097OA139nphHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA010097OA139nphHU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA010097OA139nphHU diluted at 1:50 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes: H2AFX antibody at 1.64µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa |
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