GAS1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA009264LA01HU
Article Name: GAS1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA009264LA01HU
Supplier Catalog Number: CSB-PA009264LA01HU
Alternative Catalog Number: CSB-PA009264LA01HU-100UG, CSB-PA009264LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: GAS-1 antibody, Gas1 antibody, GAS1_HUMAN antibody, Growth arrest-specific protein 1 antibody, Growth-arrest-specific protein 1 precursor antibody
Clonality: Polyclonal
UniProt: P54826
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: GAS1
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA009264LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA009264LA01HU at dilution of 1:100
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, 293 whole cell lysate
All lanes: GAS1 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Immunofluorescence staining of Hela cells with CSB-PA009264LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).