Immunofluorescent analysis of HepG2 cells using CSB-PA009262EA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA009262EA01HU at dilution of 1:100
Western Blot Positive WB detected in: U87 whole cell lysate, Mouse brain tissue, Mouse liver tissue All lanes: GARS antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84 kDa Observed band size: 84 kDa
Immunoprecipitating GARS in HL60 whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA009262EA01HU in HL60 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA009262EA01HU (8ug) + HL60 whole cell lysate (500ug) Lane 3: HL60 whole cell lysate (20ug)
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