GARS Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA009262EA01HU
Article Name: GARS Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA009262EA01HU
Supplier Catalog Number: CSB-PA009262EA01HU
Alternative Catalog Number: CSB-PA009262EA01HU-100UG, CSB-PA009262EA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Alternative Names: GARS1 antibody, GARSGlycine--tRNA ligase antibody, EC 6.1.1.14 antibody, Diadenosine tetraphosphate synthetase antibody, Ap4A synthetase antibody, EC 2.7.7.- antibody, Glycyl-tRNA synthetase antibody, GlyRS antibody, Glycyl-tRNA synthetase 1 antibody
Clonality: Polyclonal
UniProt: P41250
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: GARS
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Immunofluorescent analysis of HepG2 cells using CSB-PA009262EA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA009262EA01HU at dilution of 1:100
Western Blot
Positive WB detected in: U87 whole cell lysate, Mouse brain tissue, Mouse liver tissue
All lanes: GARS antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Immunoprecipitating GARS in HL60 whole cell lysate
Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA009262EA01HU in HL60 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA009262EA01HU (8ug) + HL60 whole cell lysate (500ug)
Lane 3: HL60 whole cell lysate (20ug)