Immunofluorescent analysis of HepG2 cells using CSB-PA008780LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA008780LA01HU at dilution of 1:100
Immunoprecipitating FNTB in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of CSB-PA008780LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA008780LA01HU (8µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
Western blot All lanes: FNTB antibody at 6µg/ml Lane 1: Hela whole cell lysate Lane 2: CEM whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 44 kDa Observed band size: 49 kDa
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