FKBP4 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA008700EA01HU
Article Name: FKBP4 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA008700EA01HU
Supplier Catalog Number: CSB-PA008700EA01HU
Alternative Catalog Number: CSB-PA008700EA01HU-100UG, CSB-PA008700EA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: 51 kDa FK506-binding protein antibody, 52 kDa FK506 binding protein antibody, 52 kDa FK506-binding protein antibody, 52 kDa FKBP antibody, 59 kDa immunophilin antibody, FK506 binding protein 4 antibody, FK506-binding protein 4 antibody, FKBP 4 antibody, FKBP 52 antibody, FKBP 59 antibody, FKBP-4 antibody, FKBP-52 antibody, FKBP4 antibody, FKBP4_HUMAN antibody, FKBP51 antibody, FKBP52 protein antibody, FKBP59 antibody, HBI antibody, Hsp 56 antibody, HSP binding immunophilin antibody, HSP-binding immunophilin antibody, Hsp56 antibody, Immunophilin FKBP52 antibody, N-terminally processed antibody, p52 antibody, p59 antibody, p59 protein antibody, Peptidyl prolyl cis trans isomerase antibody, peptidyl-prolyl cis-trans isomerase antibody, Peptidyl-prolyl cis-trans isomerase FKBP4 antibody, Peptidylprolyl cis trans isomerase antibody, PPIase antibody, PPIase FKBP4 antibody, Rotamase antibody, T cell FK506 binding protein 59kD antibody
Clonality: Polyclonal
UniProt: Q02790
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: FKBP4
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Immunofluorescence staining of Hela cells with CSB-PA008700EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA008700EA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA008700EA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate
All lanes: FKBP4 antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 59 kDa