FGF1 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA008615ESR1HU
- Images (5)
| Article Name: | FGF1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA008615ESR1HU |
| Supplier Catalog Number: | CSB-PA008615ESR1HU |
| Alternative Catalog Number: | CSB-PA008615ESR1HU-100UL, CSB-PA008615ESR1HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human, Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | Acidic fibroblast growth factor antibody, aFGF antibody, Beta endothelial cell growth factor antibody, Beta-endothelial cell growth factor antibody, ECGF antibody, ECGF beta antibody, ECGF-beta antibody, ECGFA antibody, ECGFB antibody, Endothelial Cell Growth Factor alpha antibody, Endothelial Cell Growth Factor beta antibody, FGF 1 antibody, FGF alpha antibody, Fgf1 antibody, FGF1_HUMAN antibody, FGFA antibody, Fibroblast Growth Factor 1 Acidic antibody, Fibroblast growth factor 1 antibody, GLIO703 antibody, HBGF 1 antibody, HBGF-1 antibody, HBGF1 antibody, Heparin binding growth factor 1 antibody, Heparin binding growth factor 1 precursor antibody, Heparin-binding growth factor 1 antibody |
| Clonality: | Polyclonal |
| UniProt: | P05230 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | FGF1 |
| Application Dilute: | Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:500, IF:1:50-1:200 |
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA008615ESR1HU at dilution of 1:100 |
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Immunofluorescence staining of Hela cells with CSB-PA008615ESR1HU at 1:96, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA008615ESR1HU diluted at 1:289 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA008615ESR1HU diluted at 1:289 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western blot All lanes: Fibroblast growth factor 1 antibody at 6ug/ml Lane 1: Mouse kidney tissue Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18, 7 kDa Observed band size: 18 kDa |
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