E2F1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA007340LA01HU
Article Name: E2F1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA007340LA01HU
Supplier Catalog Number: CSB-PA007340LA01HU
Alternative Catalog Number: CSB-PA007340LA01HU-100UG, CSB-PA007340LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, IP, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: Dmel\CG6376 antibody, Dmel_CG6376 antibody, drosE2F1 antibody, E(Sev-CycE)3A antibody, E(var)3-93E antibody, E2-promoter binding facto antibody, E2F 1 antibody, E2F transcription factor 1 antibody, E2F-1 antibody, E2f-PA antibody, E2f-PB antibody, E2f-PC antibody, E2F1 antibody, E2f1 E2F transcription factor 1 antibody, E2F1_HUMAN antibody, Evar(3)164 antibody, KIAA4009 antibody, l(3)07172 antibody, l(3)j3B1 antibody, l(3)j3C2 antibody, l(3)rM729 antibody, mKIAA4009 antibody, OTTHUMP00000030661 antibody, PBR3 antibody, PRB binding protein E2F 1 antibody, PRB-binding protein E2F-1 antibody, RBAP 1 antibody, RBAP-1 antibody, RBAP1 antibody, RBBP-3 antibody, RBBP3 antibody, RBP 3 antibody, RBP3 antibody, Retinoblastoma-associated protein 1 antibody, Retinoblastoma-binding protein 3 antibody, Transcription factor E2F1 antibody
Clonality: Polyclonal
UniProt: Q01094
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: E2F1
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA007340LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA007340LA01HU at dilution of 1:100
Immunoprecipitating E2F1 in 3T3 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA007340LA01HU in 3T3 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA007340LA01HU (8µg) + 3T3 whole cell lysate (500µg)
Lane 3: 3T3 whole cell lysate (10µg)
Western blot
All lanes: E2F1 antibody at 7 µg/ml + 3T3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa