DPPA5 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA007147LA01HU
- Images (4)
| Article Name: | DPPA5 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA007147LA01HU |
| Supplier Catalog Number: | CSB-PA007147LA01HU |
| Alternative Catalog Number: | CSB-PA007147LA01HU-100UG, CSB-PA007147LA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human, Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | Developmental pluripotency associated 5 antibody, Developmental pluripotency-associated 5 protein antibody, DPPA5 antibody, DPPA5_HUMAN antibody, Embryonal stem cell specific gene 1 protein antibody, Embryonal stem cell-specific gene 1 protein antibody, Esg 1 antibody, Esg-1 antibody, ESG1 antibody, hDPPA5 antibody |
| Clonality: | Polyclonal |
| UniProt: | A6NC42 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | DPPA5 |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
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Immunofluorescence staining of HepG2 cells with CSB-PA007147LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA007147LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA007147LA01HU diluted at 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: Mouse kidney tissue All lanes: DPPA5 antibody at 4.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa |
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