CYP2C19 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA006417LA01HU
Article Name: CYP2C19 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA006417LA01HU
Supplier Catalog Number: CSB-PA006417LA01HU
Alternative Catalog Number: CSB-PA006417LA01HU-100UG, CSB-PA006417LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: (R)-limonene 6-monooxygenase antibody, (S)-limonene 6-monooxygenase antibody, (S)-limonene 7-monooxygenase antibody, CP2CJ_HUMAN antibody, CPCJ antibody, CYP2C antibody, CYP2C19 antibody, CYPIIC17 antibody, CYPIIC19 antibody, Cytochrome P-450 II C antibody, Cytochrome P450 2C19 antibody, Cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 19 antibody, Cytochrome P450-11A antibody, Cytochrome P450-254C antibody, Flavoprotein-linked monooxygenase antibody, Mephenytoin 4 hydroxylase antibody, Mephenytoin 4-hydroxylase antibody, Microsomal monooxygenase antibody, P450-11A antibody, P45011A antibody, P450C2C antibody, S-mephenytoin 4-hydroxylase antibody, Xenobiotic monooxygenase antibody
Clonality: Polyclonal
UniProt: P33261
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: CYP2C19
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:300-1:500, IF:1:50-1:200
IHC image of CSB-PA006417LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA006417LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-PA006417LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: Mouse kidney tissue
All lanes: CYP2C19 antibody at 4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa