CRYAB Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA006008HA01HU
- Images (4)
| Article Name: | CRYAB Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA006008HA01HU |
| Supplier Catalog Number: | CSB-PA006008HA01HU |
| Alternative Catalog Number: | CSB-PA006008HA01HU-100UG, CSB-PA006008HA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | AACRYA antibody, Alpha B crystallin antibody, Alpha crystallin B chain antibody, Alpha(B)-crystallin antibody, Alpha-crystallin B chain antibody, CRYA2 antibody, Cryab antibody, CRYAB_HUMAN antibody, Crystallin alpha B antibody, Crystallin alpha polypeptide 2 antibody, CTPP2 antibody, Heat shock 20 kD like protein antibody, Heat shock protein beta 5 antibody, Heat shock protein beta-5 antibody, HspB5 antibody, Renal carcinoma antigen NY REN 27 antibody, Renal carcinoma antigen NY-REN-27 antibody, Rosenthal fiber component antibody |
| Clonality: | Polyclonal |
| UniProt: | P02511 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | CRYAB |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
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IHC image of CSB-PA006008HA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: Rat heart tissue, Mouse heart tissue All lanes: CRYAB antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa |
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Immunofluorescence staining of U251 cells with CSB-PA006008HA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA006008HA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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