CPS1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA005913LA01HU
Article Name: CPS1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA005913LA01HU
Supplier Catalog Number: CSB-PA005913LA01HU
Alternative Catalog Number: CSB-PA005913LA01HU-100UG, CSB-PA005913LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: Carbamoyl phosphate synthase [ammonia] antibody, Carbamoyl phosphate synthase [ammonia] mitochondrial antibody, Carbamoyl phosphate synthase antibody, Carbamoyl phosphate synthetase 1 antibody, Carbamoyl phosphate synthetase 1 mitochondrial antibody, Carbamoyl phosphate synthetase I antibody, Carbamoyl-phosphate synthase [ammonia] antibody, Carbamoyl-phosphate synthetase I antibody, Carbamoylphosphate synthase antibody, Carbamoylphosphate synthetase 1 antibody, Carbamoylphosphate synthetase I antibody, CPS 1 antibody, Cps1 antibody, CPSase 1 antibody, CPSase I antibody, CPSASE1 antibody, CPSM_HUMAN antibody, mitochondrial antibody, MS738 antibody
Clonality: Polyclonal
UniProt: P31327
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purity: Antigen Affinity Purified
Form: Liquid
Target: CPS1
Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200
IHC image of CSB-PA005913LA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: U251 whole cell lysate(30µg), K562 whole cell lysate(30µg), HT29 whole cell lysate(30µg)
All lanes: CPS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 165,117,166 kDa
Observed band size: 165 kDa
Exposure time: 120s