CNTFR Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA005684EA01HU
Article Name: CNTFR Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA005684EA01HU
Supplier Catalog Number: CSB-PA005684EA01HU
Alternative Catalog Number: CSB-PA005684EA01HU-100UG, CSB-PA005684EA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: Ciliary neurotrophic factor receptor alpha antibody, Ciliary neurotrophic factor receptor antibody, Ciliary neurotrophic factor receptor subunit alpha antibody, CNTF Receptor alpha antibody, CNTF receptor subunit alpha antibody, CNTFR Alpha antibody, CNTFR antibody, CNTFR-alpha antibody, CNTFR_HUMAN antibody
Clonality: Polyclonal
UniProt: P26992
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: CNTFR
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA005684EA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA005684EA01HU at dilution of 1:100
Western blot
All lanes: CNTFR antibody at 2µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Immunofluorescence staining of SH-SY5Y cells with CSB-PA005684EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).