CLTCL1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA005594LA01HU
Article Name: CLTCL1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA005594LA01HU
Supplier Catalog Number: CSB-PA005594LA01HU
Alternative Catalog Number: CSB-PA005594LA01HU-100UG, CSB-PA005594LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: CLTCL1 antibody, CLH22 antibody, CLTCL antibody, CLTDClathrin heavy chain 2 antibody, Clathrin heavy chain on chromosome 22 antibody, CLH-22 antibody
Clonality: Polyclonal
UniProt: P53675
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: CLTCL1
Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunofluorescence staining of A549 cells with CSB-PA005594LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA005594LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA005594LA01HU at dilution of 1:100
Western blot
All lanes: CLTCL1 antibody at 2µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: A431 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 188, 181 kDa
Observed band size: 188 kDa