CHAT Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA005314LA01HU
- Images (4)
| Article Name: | CHAT Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA005314LA01HU |
| Supplier Catalog Number: | CSB-PA005314LA01HU |
| Alternative Catalog Number: | CSB-PA005314LA01HU-100UG, CSB-PA005314LA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | Acetyl CoA choline O acetyltransferase antibody, Acetyl CoA:choline O acetyltransferase antibody, ChAT antibody, CHOACTase antibody, Choline acetylase antibody, choline acetyltransferase antibody, Choline O acetyltransferase antibody, Choline O-acetyltransferase antibody, CLAT_HUMAN antibody, CMS1A antibody, CMS1A2 antibody, EC 2.3.1.6 antibody, OTTHUMP00000019583 antibody, OTTHUMP00000019584 antibody |
| Clonality: | Polyclonal |
| UniProt: | P28329 |
| Buffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | CHAT |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
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Immunofluorescent analysis of HepG2 cells using CSB-PA005314LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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IHC image of CSB-PA005314LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, Mouse liver tissue All lanes: CHAT antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83, 75, 71 kDa Observed band size: 75 kDa |
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Western Blot Positive WB detected in: Mouse Brain tissue lysate,Rat Brain tissue lysate All lanes: CHAT antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47-83 kDa Observed band size: 70 kDa |
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