CENPK Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA005214ESR1HU
Article Name: CENPK Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA005214ESR1HU
Supplier Catalog Number: CSB-PA005214ESR1HU
Alternative Catalog Number: CSB-PA005214ESR1HU-100UL, CSB-PA005214ESR1HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, IP, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: CENPK antibody, ICEN37 antibody, FKSG14 antibody, Centromere protein K antibody, CENP-K antibody, Interphase centromere complex protein 37 antibody, Protein AF-5alpha antibody, p33 antibody
Clonality: Polyclonal
UniProt: Q9BS16
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purity: Antigen Affinity Purified
Form: Liquid
Target: CENPK
Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA005214ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA005214ESR1HU at dilution of 1:100
Western blot
All lanes: CENPK antibody at 3.98ug/ml
Lane 1: PC-3 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Immunoprecipitating CENPK in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA005214ESR1HU in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA005214ESR1HU (8ug) + Hela whole cell lysate (500ug)
Lane 3: Hela whole cell lysate (10ug)