CDK2 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA005061LA01HU
- Images (5)
| Article Name: | CDK2 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA005061LA01HU |
| Supplier Catalog Number: | CSB-PA005061LA01HU |
| Alternative Catalog Number: | CSB-PA005061LA01HU-100UG, CSB-PA005061LA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, IP, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | Cdc2 related protein kinase antibody, cdc2-related protein kinase antibody, CDC28 antibody, CDC2A antibody, Cdk 2 antibody, CDK1 antibody, CDK2 antibody, CDK2_HUMAN antibody, CDKN2 antibody, Cell devision kinase 2 antibody, Cell division protein kinase 2 antibody, Cyclin dependent kinase 2 antibody, cyclin dependent kinase 2-alpha antibody, Cyclin-dependent kinase 2 antibody, kinase Cdc2 antibody, MPF antibody, p33 protein kinase antibody, p33(CDK2) antibody |
| Clonality: | Polyclonal |
| UniProt: | P24941 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | CDK2 |
| Application Dilute: | Recommended dilution: WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:200-1:500, IP:1:200-1:2000 |
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Immunofluorescence staining of HepG2 cells with CSB-PA005061LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunoprecipitating CDK2 in Hela whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of CSB-PA005061LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: CSB-PA005061LA01HU (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg) |
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Western Blot Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes: CDK2 antibody at 2.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 31 kDa Observed band size: 34 kDa |
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IHC image of CSB-PA005061LA01HU diluted at 1:800 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA005061LA01HU diluted at 1:800 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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