CA1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA004364HA01HU
Article Name: CA1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA004364HA01HU
Supplier Catalog Number: CSB-PA004364HA01HU
Alternative Catalog Number: CSB-PA004364HA01HU-100UG, CSB-PA004364HA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, IP, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: CA 1 antibody, CA I antibody, CA-I antibody, CA1 antibody, CAB antibody, CAH1_HUMAN antibody, CAI antibody, CAN antibody, Car 1 antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase A antibody, Carbonic anhydrase B antibody, Carbonic anhydrase B, formerly antibody, Carbonic anhydrase I antibody, Carbonic dehydratase antibody, ECK0125 antibody, JW0122 antibody, yadF antibody
Clonality: Polyclonal
UniProt: P00915
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: CA1
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Immunofluorescent analysis of A549 cells using CSB-PA004364HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA004364HA01HU at dilution of 1:100
Immunoprecipitating CA1 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA004364HA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA004364HA01HU (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (20µg)
Western Blot
Positive WB detected in: K562 whole cell lysate, Mouse spleen tissue, Mouse kidney tissue
All lanes: CA1 antibody at 6.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa