Immunofluorescent analysis of A549 cells using CSB-PA004364HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA004364HA01HU at dilution of 1:100
Immunoprecipitating CA1 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA004364HA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA004364HA01HU (8µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)
Western Blot Positive WB detected in: K562 whole cell lysate, Mouse spleen tissue, Mouse kidney tissue All lanes: CA1 antibody at 6.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
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