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Immunofluorescence staining of Hela cells with CSB-PA00414A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00414A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA00414A0Rb at dilution of 1:100
Western blot All lanes: COX5A antibody at 2µg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa
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