ASPH Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA002226HA01HU
- Images (4)
| Article Name: | ASPH Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA002226HA01HU |
| Supplier Catalog Number: | CSB-PA002226HA01HU |
| Alternative Catalog Number: | CSB-PA002226HA01HU-100UG, CSB-PA002226HA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human, Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | ASPH antibody, BAHAspartyl/asparaginyl beta-hydroxylase antibody, EC 1.14.11.16 antibody, Aspartate beta-hydroxylase antibody, ASP beta-hydroxylase antibody, Peptide-aspartate beta-dioxygenase antibody |
| Clonality: | Polyclonal |
| UniProt: | Q12797 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | ASPH |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: ASPH antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 35, 26, 24, 34, 84, 30, 22, 29, 33 kDa Observed band size: 86 kDa |
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Immunofluorescence staining of Hela cells with CSB-PA002226HA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA002226HA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA002226HA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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