ATP6V1G1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA00135A0RB
Article Name: ATP6V1G1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA00135A0RB
Supplier Catalog Number: CSB-PA00135A0Rb
Alternative Catalog Number: CSB-PA00135A0RB-100UG, CSB-PA00135A0RB-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: ATP 6G antibody, ATP 6J antibody, ATP6G antibody, ATP6G1 antibody, ATP6GL antibody, Atp6v1g1 antibody, ATPase H+ transporting lysosomal (vacuolar proton pump) member J antibody, ATPase H+ transporting lysosomal 13kD V1 subunit G isoform 1 antibody, ATPase H+ transporting lysosomal 13kDa V1 subunit G1 antibody, ATPase H+ transporting lysosomal member J antibody, ATPase H+ transporting lysosomal subunit G isoform 1 antibody, DKFZp547P234 antibody, V ATPase 13 kDa subunit 1 antibody, V ATPase G subunit 1 antibody, V-ATPase 13 kDa subunit 1 antibody, V-ATPase subunit G 1 antibody, V-type proton ATPase subunit G 1 antibody, Vacuolar ATP synthase subunit G 1 antibody, Vacuolar ATP synthase subunit G1 antibody, Vacuolar ATP synthase subunit M16 antibody, Vacuolar H+ ATPase G1 antibody, Vacuolar H+ ATPase subunit G 1 antibody, Vacuolar proton pump G subunit 1 antibody, Vacuolar proton pump subunit G 1 antibody, Vacuolar proton pump subunit M16 antibody, VATG1_HUMAN antibody, Vma 10 antibody, Vma10 antibody
Clonality: Polyclonal
UniProt: O75348
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: ATP6V1G1
Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00135A0Rb at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00135A0Rb at dilution of 1:100
Immunofluorescence staining of PC-3 cells with CSB-PA00135A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot
All lanes: V-type proton ATPase subunit G 1 antibody at 2µg/ml + 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa