ADH1B Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA001354HA01HU
Article Name: ADH1B Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA001354HA01HU
Supplier Catalog Number: CSB-PA001354HA01HU
Alternative Catalog Number: CSB-PA001354HA01HU-100UG, CSB-PA001354HA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: ADH beta subunit antibody, ADH1B antibody, ADH1B_HUMAN antibody, ADH2 antibody, Alcohol dehydrogenase 1B antibody, alcohol dehydrogenase 2 (class I), beta polypeptide antibody, Alcohol dehydrogenase 2 antibody, Alcohol dehydrogenase subunit beta antibody, Aldehyde reductase antibody, DKFZp686C06125 antibody, OTTHUMP00000220192 antibody
Clonality: Polyclonal
UniProt: P00325
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: ADH1B
Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA001354HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA001354HA01HU at dilution of 1:100
Western blot
All lanes: ADH1B antibody at 2µg/ml + HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 40, 36 kDa
Observed band size: 40 kDa
Immunofluorescence staining of HepG2 cells with CSB-PA001354HA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).