LAG3 Monoclonal Antibody, IgG1, Clone: [8B12E10], Unconjugated, Mouse
Catalog Number:
CSB-MA012719A0M
- Images (4)
| Article Name: | LAG3 Monoclonal Antibody, IgG1, Clone: [8B12E10], Unconjugated, Mouse |
| Biozol Catalog Number: | CSB-MA012719A0M |
| Supplier Catalog Number: | CSB-MA012719A0m |
| Alternative Catalog Number: | CSB-MA012719A0M-100UL, CSB-MA012719A0M-50UL |
| Manufacturer: | Cusabio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, FC, IHC, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | CD223 antibody, CD223 antigen antibody, FDC protein antibody, LAG-3 antibody, Lag3 antibody, LAG3_HUMAN antibody, Lymphocyte activating 3 antibody, Lymphocyte activation gene 3 antibody, Lymphocyte activation gene 3 protein antibody, Protein FDC antibody |
| Clonality: | Monoclonal |
| Clone Designation: | [8B12E10] |
| Isotype: | IgG1 |
| UniProt: | P18627 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | LAG3 |
| Application Dilute: | Recommended dilution: WB: 1:1000-1:5000, IHC: 1:200-1:1000, FC: 1:50-1:200 |
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Overlay Peak curve showing A549 cells stained with CSB-MA012719A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106 cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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IHC image of CSB-MA012719A0m diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-MA012719A0m diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: 1 lane: Recombinant proteins with LAG3, 2 lane: 293F whole cell lysate transfected with LAG3 All lanes: LAG3 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 76, 88 KDa Observed band size: 76, 88 KDa Exposure time:5min |
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