Phospho-Smad2 (Ser467) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB783427
- Images (9)
| Article Name: | Phospho-Smad2 (Ser467) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB783427 |
| Supplier Catalog Number: | orb783427 |
| Alternative Catalog Number: | BYT-ORB783427-50,BYT-ORB783427-100,BYT-ORB783427-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser467 |
| Conjugation: | Unconjugated |
| Alternative Names: | SMAD2 (p-T220), p-SMAD2, phospho-SMAD2, CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2, 7120426M23Rik, Smad-2, mMad2, SMAD2_HUMAN, SMAD2, MAD homolog 2, Mothers against DPP homolog 2, Mad-related protein 2 (hMAD-2), SMAD family member 2 (SMAD 2 | Smad2 | hSMAD2), SMAD2_MOUSE, Mad-related protein 2 (mMad2), SMAD family member 2 (SMAD 2 | Smad2), SMAD2_RAT, Mad-related protein 2, |
| Phospho-Smad2 (Ser467) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 58 kDa |
| UniProt: | Q15796 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SMAD2 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (phospho-Smad2 (Ser467)) polyclonal Antibody, Unconjugated (orb783427) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (Human glioblastoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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IHC-P analysis of rat placenta using phospho-Smad2 Ser467 Polyclonal Antibody at 1:200 dilution. |
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