COL4A3 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB763112
Article Name: COL4A3 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB763112
Supplier Catalog Number: orb763112
Alternative Catalog Number: BYT-ORB763112-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC
Species Reactivity: Human
Immunogen: E.coli-derived human COL4A3 recombinant protein (Position: P1426-H1670).
Conjugation: Unconjugated
Alternative Names: COL4A3, Tumstatin, Goodpasture antigen, Collagen alpha-3(IV) chain, ATS2, ATS3, ATS3A, ATS3B, BFH2
Anti-COL4A3 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Human.
Clonality: Polyclonal
Concentration: 500 µg/ml
Molecular Weight: 130 kDa
UniProt: Q01955
Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Form: Lyophilized
Target: Collagen alpha-3
Application Dilute: Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human ELISA, 0.1-0.5 µg/ml, -
IHC analysis of COL4A3 using anti-COL4A3 antibody. COL4A3 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-COL4A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of COL4A3 using anti-COL4A3 antibody. COL4A3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-COL4A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of COL4A3 using anti-COL4A3 antibody. COL4A3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-COL4A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of COL4A3 using anti-COL4A3 antibody. COL4A3 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-COL4A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of COL4A3 using anti-COL4A3 antibody. COL4A3 was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-COL4A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of COL4A3 using anti-COL4A3 antibody. COL4A3 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-COL4A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.