Recombinant human TNFSF11 Protein with N-terminal Human Fc tag
Molecular Weight:
The protein has a predicted molecular mass of 48.4 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TNFSF11 is approximately 55-70 kDa due to glycosylation.
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Purity:
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form:
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target:
TNFSF11
Human CD27 Protein, hFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CD27, hFc tagged protein (orb689459) can bind Anti-CD27 Neutralizing antibody orb689323 in a linear range of 3.2-80.0 ng/ml.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CD27, hFc tagged protein (orb689459) can bind Human CD70, mFc-His tagged protein orb689401 in a linear range of 3.2-400 ng/ml.
Flow cytometry analysis with 15 µg/mL Human CD27 Protein, hFc tag (orb689459) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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