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EGFR Rabbit Polyclonal Antibody, Unconjugated

EGFR Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB704502

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Article Name:	EGFR Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number:	BYT-ORB704502
Supplier Catalog Number:	orb704502
Alternative Catalog Number:	BYT-ORB704502-50,BYT-ORB704502-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC-Fr, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	KLH conjugated synthetic peptide derived from human EGFR
Conjugation:	Unconjugated
Alternative Names:	ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA, 9030024J15Rik, Errb1, Wa5, wa-2, wa2, ErbB-1, EGFR_HUMAN, EGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, 2.7.10.1, EGFR_MOUSE,

EGFR Rabbit Polyclonal Antibody

Clonality:	Polyclonal
Concentration:	1mg/ml
Molecular Weight:	175 kDa
Pubmed:	41613010
UniProt:	P00533
Buffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:	Liquid
Target:	EGFR

Application Dilute:

WB=1:1000-5000, IHC-F=1:100-500, IF=1:100-500, IHC-P=1:100-500

	25 ug total protein per lane of various lysates probed with EGFR polyclonal antibody, unconjugated (orb704502) at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
	Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (orb704502) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (orb704502) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (orb704502) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human MDA-MB-231 cell lysates, Primary: Anti-EGFR (orb704502) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 175 kDa, Observed band size: 170 kDa.
	IHC-P analysis of rat placenta using EGFR Polyclonal Antibody at 1:200 dilution.
	IHC-P analysis of human gastric carcinoma using EGFR Polyclonal Antibody at 1:200 dilution.

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