MMP9 Recombinant Rabbit Monoclonal Antibody, Clone: [7D6], Unconjugated

Catalog Number: BYT-ORB704499
Article Name: MMP9 Recombinant Rabbit Monoclonal Antibody, Clone: [7D6], Unconjugated
Biozol Catalog Number: BYT-ORB704499
Supplier Catalog Number: orb704499
Alternative Catalog Number: BYT-ORB704499-25,BYT-ORB704499-50,BYT-ORB704499-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthesized peptide derived from human MMP9 (100-165/707aa)
Conjugation: Unconjugated
Alternative Names: CLG4B, GELB, MANDP2, MMP-9, B/MMP9, Gel B, pro-MMP-9, MMP9_CANLF, MMP9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B (GELB), 3.4.24.35, MMP9_HUMAN, MMP9_MOUSE,
MMP9 Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [7D6]
Molecular Weight: 100 kDa
Pubmed: 40581980
UniProt: P14780
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: Mmp9
Application Dilute: WB=1:2000-20000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP9) Monoclonal Antibody, Unconjugated (orb704499) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP9) Monoclonal Antibody, Unconjugated (orb704499) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Normal human U-2 OS cell lysates, Lane 2: U-2 OS treated with 200nM TPA for 48 hours, Lane 3: Normal human MDA-MB-231 cell lysates, Lane 4: MDA-MB-231 serum starved overnight, then treated with 200nM TPA for 24 hours, Primary: Anti-MMP9 (orb704499) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kDa, Observed band size: 82 kDa.
Sample: Lane 1: Spleen (Rat) Lysate at 40 ug, Lane 2: Lung (Rat) Lysate at 40 ug, Primary: Anti-MMP9 (orb704499) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 92/82-85 kD, Observed band size: 90 kD.
IHC-P analysis of human gastric carcinoma using MMP9 Monoclonal Antibody at 1:200 dilution.
Western blot:Lane 1:Spleen (Rat),Lane 2:Lung (Rat),Primary:Anti-MMP9 at 1:1000 dilution.



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