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Human IgM Recombinant Rabbit Monoclonal Antibody, Clone: [4C2], Unconjugated

Human IgM Recombinant Rabbit Monoclonal Antibody, Clone: [4C2], Unconjugated

Catalog Number: BYT-ORB704301

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Article Name:	Human IgM Recombinant Rabbit Monoclonal Antibody, Clone: [4C2], Unconjugated
Biozol Catalog Number:	BYT-ORB704301
Supplier Catalog Number:	orb704301
Alternative Catalog Number:	BYT-ORB704301-50,BYT-ORB704301-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC-Fr, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Human IgM
Conjugation:	Unconjugated
Alternative Names:	human Immunoglobulin M, Human IgM,

Human IgM Recombinant Rabbit Monoclonal Antibody

Clonality:	Recombinant
Concentration:	1mg/ml
Clone Designation:	[4C2]
Molecular Weight:	900 kDa
Buffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:	Liquid

Application Dilute:

WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

	Paraformaldehyde-fixed, paraffin embedded (human spleen tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Human IgM) Monoclonal Antibody, Unconjugated (orb704301) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Human IgM) Monoclonal Antibody, Unconjugated (orb704301) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Human IgM) Monoclonal Antibody, Unconjugated (orb704301) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Sample: Lane 1: human plasma, Primary: Anti-Human IgM (orb704301) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 900 kD, Observed band size: 75 kD.
	Sample: Lane 1: Human Serum, Primary: Anti-Human IgM (orb704301) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 900 kDa, Observed band size: 75 kDa.
	IHC-P analysis of human spleen tissue using Human IgM Monoclonal Antibody at 1:50 dilution.
	Western blot:Lane 1:human plasma,Primary:Anti-Human IgM at 1:500 dilution.

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