Cytochrome P450 17A1 Recombinant Rabbit Monoclonal Antibody, Clone: [2F7], Unconjugated
Catalog Number:
BYT-ORB704167
- Images (7)
| Article Name: | Cytochrome P450 17A1 Recombinant Rabbit Monoclonal Antibody, Clone: [2F7], Unconjugated |
| Biozol Catalog Number: | BYT-ORB704167 |
| Supplier Catalog Number: | orb704167 |
| Alternative Catalog Number: | BYT-ORB704167-50,BYT-ORB704167-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human Cytochrome P450 17A1 (101-220aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CYP17, CYPXVII, CPT7, P450C17, S17AH, CP17A_FELCA, CYP17A1, 17-alpha-hydroxyprogesterone aldolase, Cytochrome P450 17A1, Cytochrome P450-C17 (Cytochrome P450c17), Steroid 17-alpha-monooxygenase, 1.14.14.19, CP17A_PANTR, CP17A_MESAU, CP17A_HORSE, CP17A_HUMAN, |
| Cytochrome P450 17A1 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [2F7] |
| Molecular Weight: | 57 kDa |
| UniProt: | P05093 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CYP17A1 |
| Application Dilute: | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100 |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytochrome P450 17A1) Monoclonal Antibody, Unconjugated (orb704167) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytochrome P450 17A1) Monoclonal Antibody, Unconjugated (orb704167) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytochrome P450 17A1) Monoclonal Antibody, Unconjugated (orb704167) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytochrome P450 17A1) Monoclonal Antibody, Unconjugated (orb704167) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Adrenal gland (Mouse) Lysate at 40 ug, Lane 2: Uterus (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Testis (Rat) Lysate at 40 ug, Lane 5: Adrenal gland (Rat) Lysate at 40 ug, Primary: Anti-Cytochrome P450 17A1 (orb704167) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 55 kD. |
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IHC-P analysis of rat kidney using Cytochrome P450 17A1 Monoclonal Antibody at 1:200 dilution. |
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IHC-P analysis of mouse kidney using Cytochrome P450 17A1 Monoclonal Antibody at 1:200 dilution. |
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