Ephrin B2 Recombinant Rabbit Monoclonal Antibody, Clone: [3A7], Unconjugated
Catalog Number:
BYT-ORB704166
- Images (7)
| Article Name: | Ephrin B2 Recombinant Rabbit Monoclonal Antibody, Clone: [3A7], Unconjugated |
| Biozol Catalog Number: | BYT-ORB704166 |
| Supplier Catalog Number: | orb704166 |
| Alternative Catalog Number: | BYT-ORB704166-50,BYT-ORB704166-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Ephrin B2 |
| Conjugation: | Unconjugated |
| Alternative Names: | EPLG5, HTKL, Htk-L, LERK5, ephrin-B2, ELF-2, Epl5, LERK-5, NLERK-1, EFNB2_HUMAN, EFNB2, EPH-related receptor tyrosine kinase ligand 5 (LERK-5), HTK ligand (HTK-L), EFNB2_MOUSE, Elf2, |
| Ephrin B2 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [3A7] |
| Molecular Weight: | 34 kDa |
| UniProt: | P52799 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | EFNB2 |
| Application Dilute: | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
|
|
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (orb704166) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (orb704166) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (orb704166) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (orb704166) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-Ephrin B2 (orb704166) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45-50 kD, Observed band size: 47 kD. |
|
|
IHC-P analysis of Human kidney using Ephrin B2 Monoclonal Antibody at 1:200 dilution. |
|
|
IHC-P analysis of rat uterus using Ephrin B2 Monoclonal Antibody at 1:200 dilution. |
Product Guarantee and Expert Support
