Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Purity:
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form:
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target:
ICOS
Human CTLA-4 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human B7-1, hFc tagged protein orb689457 can bind Human CTLA4, mFc-His tagged protein (orb689378) in a linear range of 0.048-2.094 ng/ml.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human B7-2, hFc tagged protein orb689458 can bind Human CTLA4, mFc-His tagged protein (orb689378) in a linear range of 0.048-2.694 ng/ml.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CTLA-4, mFc-His tagged protein (orb689378) can bind Anti-CTLA-4 Neutralizing antibody orb689327 in a linear range of 0.13-16.0 ng/ml.
Flow cytometry analysis with 1 µg/mL Human CTLA4 Protein, mFc-His tag (orb689378) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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