PDHA1 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6669
- Images (6)
| Article Name: | PDHA1 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6669 |
| Supplier Catalog Number: | orb6669 |
| Alternative Catalog Number: | BYT-ORB6669-100,BYT-ORB6669-200,BYT-ORB6669-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PDHA1 (251-350/388aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | E1alpha, PDHA, PDHAD, PDHCE1A, PHE1A, Pdha-1, ODPA_BOVIN, PDHA1, PDHE1-A type I, 1.2.4.1, ODPA_HUMAN, ODPA_MOUSE, ODPA_RAT, |
| PDHA1 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 43 kDa |
| UniProt: | P08559 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PDHA1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (PDHA1) Polyclonal Antibody, Unconjugated (orb6669) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (PDHA1) Polyclonal Antibody, Unconjugated (orb6669) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PDHA1) Polyclonal Antibody, Unconjugated (orb6669) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (PDHA1) Polyclonal Antibody, Unconjugated (orb6669) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-PDHA1 (orb6669) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD. |
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Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-PDHA1 (orb6669) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD. |
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