Phospho-p73 (Tyr99) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6609
Article Name: Phospho-p73 (Tyr99) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6609
Supplier Catalog Number: orb6609
Alternative Catalog Number: BYT-ORB6609-50,BYT-ORB6609-100,BYT-ORB6609-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human p73 around the phosphorylation site of Tyr99 SP(p-Y)AQ
Conjugation: Unconjugated
Alternative Names: TP73 | p73 (p-Y99), p-p73, phospho-p73, CILD47, P73, TAp73, Tp73, deltaNp73, Trp73, P73_HUMAN, p53-like transcription factor, p53-related protein, P73_MOUSE,
Phospho-p73 (Tyr99) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 70 kDa
UniProt: O15350
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: TP73
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Application Notes: Modification: Phosphorylated
Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-p73 (Tyr99)) Polyclonal Antibody, Unconjugated (orb6609) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-p73 (Tyr99)) Polyclonal Antibody, Unconjugated (orb6609) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat trachea), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-p73 (Tyr99)) Polyclonal Antibody, Unconjugated (orb6609) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Esophagus tissue lysates, Lane 2: Rat Esophagus tissue lysates, Primary: Anti-Phospho-p73 (Tyr99) (orb6609) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kDa, Observed band size: 75 kDa.
Tissue/Cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-p73 (Tyr99) Polyclonal Antibody, Unconjugated (orb6609) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical staining of human placenta tissue using p73 (phospho-Tyr99) antibody.



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