Nur77 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6550
- Images (6)
| Article Name: | Nur77 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6550 |
| Supplier Catalog Number: | orb6550 |
| Alternative Catalog Number: | BYT-ORB6550-50,BYT-ORB6550-100,BYT-ORB6550-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Nur77/GFRP (351-450/598aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3, Gfrp, Hbr-1, Hbr1, NGFI-B, NUR77-1, NUR77-2, TIS1, NR4A1_HUMAN, NR4A1, Early response protein NAK1, Nuclear hormone receptor NUR/77 (Nur77), Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, NAK1, NR4A1_MOUSE, Nuclear hormone receptor NUR/77, Nuclear protein N10, NR4A1_RAT, Nerve growth factor-induced protein I-B (NGFI-B), |
| Nur77 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 66 kDa |
| UniProt: | P22736 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | NR4A1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Immunohistochemical staining of rat brain tissue using Nur77 antibody. |
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Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Nur77 antibody (orb6550), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nur77) Polyclonal Antibody, Unconjugated (orb6550) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-Nur77 (orb6550) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 66 kDa, Observed band size: 73 kDa. |
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Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Nur77 (orb6550) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 66 kD, Observed band size: 66 kD. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Nur77 Polyclonal Antibody, Unconjugated (orb6550) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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