YAP1 Recombinant Rabbit Monoclonal Antibody, Clone: [1G2], Unconjugated
Catalog Number:
BYT-ORB612234
- Images (6)
| Article Name: | YAP1 Recombinant Rabbit Monoclonal Antibody, Clone: [1G2], Unconjugated |
| Biozol Catalog Number: | BYT-ORB612234 |
| Supplier Catalog Number: | orb612234 |
| Alternative Catalog Number: | BYT-ORB612234-25,BYT-ORB612234-50,BYT-ORB612234-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | A synthesized peptide derived from human YAP1 (350-410aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | COB1, YAP, YAP-1, YAP2, YAP65, YKI, YAP1_HUMAN, YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, |
| YAP1 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [1G2] |
| Molecular Weight: | 65 kDa |
| UniProt: | P46937 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | YAP1 |
| Application Dilute: | WB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1ug/Test |
|
|
25 ug total protein per lane of various lysates probed with YAP1 monoclonal antibody, unconjugated (orb612234) at 1:2000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |
|
|
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (YAP1) Monoclonal Antibody, Unconjugated (orb612234) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
|
|
HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (YAP1) Monoclonal Antibody, Unconjugated (orb612234) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (YAP1) Monoclonal Antibody, Unconjugated (orb612234) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (YAP1) Monoclonal Antibody, Unconjugated (orb612234) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (YAP1) Monoclonal Antibody, Unconjugated (orb612234) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Product Guarantee and Expert Support
