Phospho-PYK2 (Tyr881) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6034
Article Name: Phospho-PYK2 (Tyr881) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6034
Supplier Catalog Number: orb6034
Alternative Catalog Number: BYT-ORB6034-50,BYT-ORB6034-100,BYT-ORB6034-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthesised phosphopeptide derived from human Pyk2 around the phosphorylation site of Tyr881 LV(p-Y)LN
Conjugation: Unconjugated
Alternative Names: PTK2B | PYK2 (p-Y881), p-PYK2, phospho-PYK2, CADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK, CAKbeta, E430023O05Rik, CAK beta, FAK2_HUMAN, PTK2B, Calcium-dependent tyrosine kinase (CADTK), Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta (CAK-beta | CAKB), Focal adhesion kinase 2 (FADK 2), Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase (RAFTK), 2.7.10.2, FAK2_MOUSE, FAK2_RAT,
Phospho-PYK2 (Tyr881) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 116 kDa
UniProt: Q14289
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: PTK2B
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Application Notes: Modification: Phosphorylated
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PYK2 (Tyr881)) Polyclonal Antibody, Unconjugated (orb6034) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-phospho-PYK2 (Tyr881) (orb6034) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 116 kD, Observed band size: 116 kD.
Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-phospho-PYK2 (Tyr881) (orb6034) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 116 kD, Observed band size: 116 kD.
Tissue/Cell: Rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-PYK2 (Tyr881) Polyclonal Antibody, Unconjugated (orb6034) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical staining of rat brain tissue using Pyk2 (phospho-Tyr881) antibody.
Immunohistochemical staining of Rat kidney tissue using Pyk2 (phospho-Tyr881) antibody.



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